On Tosoh G 8, HbE induced an extra peak on chromatogram, and significantly lower results were reported. Elevated HbF induced false HbA 1c detection on Capillarys 2FP (> 10%), Tosoh G 8 (> 30%), Premier Hb9210 (> 15%), and Roche c501 (> 5%). Very high levels of triglycerides and cholesterol (≥ 15.28 and ≥ 8.72 mmol/L, respectively) led to falsely low HbA 1c concentrations on Roche c501.
Method comparison showed a great correlation and agreement between methods. The analytical bias of the four systems against the IFCC targets was less than ± 6% (NGSP units), indicating a good accuracy. Linearity analysis for each analyzer had achieved a good correlation coefficient (R 2 > 0.99) over the entire range tested. A good precision was shown at both low and high HbA 1c levels on all four systems, with all individual CVs below 2% (IFCC units) or 1.5% (NGSP units). A comparative evaluation of the analytical performances (precision, linearity, accuracy, method comparison, and interferences including bilirubin, triglyceride, cholesterol, labile HbA 1c (LA 1c ), vitamin C, aspirin, fetal haemoglobin (HbF), and haemoglobin E (Hb E)) were performed on Capillarys 2 Flex Piercing (Capillarys 2FP) (Sebia, France), Tosoh HLC- 723 G 8 ( Tosoh G 8) ( Tosoh, Japan), Premier Hb9210 (Trinity Biotech, Ireland) and Roche Cobas c501 (Roche c501) (Roche Diagnostics, Germany). Therefore, the reliability and comparability among different analytical methods for its detection have become very important. Haemoglobin A 1c (HbA 1c ) is widely used in the management of diabetes. Wu, Xiaobin Chao, Yan Wan, Zemin Wang, Yunxiu Ma, Yan Ke, Peifeng Wu, Xinzhong Xu, Jianhua Zhuang, Junhua Huang, Xianzhang A comparative evaluation of the analytical performances of Capillarys 2 Flex Piercing, Tosoh HLC- 723 G 8, Premier Hb9210, and Roche Cobas c501 Tina-quant Gen 2 analyzers for HbA1c determination.
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